Method for proving fertility improving or contraceptive agents

摘要

Detecting changes in cellular messengers (A) in sperm, using a stopped-flow measuring device, comprises: (a) mixing sperm and an active compound (I); and (b) detecting the changes by a stopped- or quenched-flow measurement, with an interval no longer than 5-1000 ms between steps (a) and (b). Independent claims are also included for the following: (1) method for detecting motility or a change in swimming behavior of sperm; and (2) cAMP or cGMP covalently linked to [6,7-bis(ethoxycarbonylmethoxy)coumarin-4-yl]methyl, and Rescat covalently linked with [S-(4,5-dimethoxy-2- nitrobenzyl-Cys8]. ACTIVITY : Antiinfertility; Contraceptive. MECHANISM OF ACTION : None given.