THERMAL DENATURATION SCREENING ASSAY TO IDENTIFY CANDIDATE COMPOUNDS FOR PREVENTION AND TREATMENT OF PARKINSON'S DISEASE

摘要

The invention provides highthroughput screening assays to identify agents useful for treatment of Parkinson's Disease. In one embodiment the assay includes exposing a plurality of test samples, each containing a test compound and parkin protein, to thermal destabilization conditions and determining parkin ligase activity in the test samples relative to a control sample not containing a test agent. A test agent contained in a test sample in which parkin ligase activity exceeds the ligase activity in said control sample is identified as a candidate compound for treatment of Parkinson's Disease.