METHOD FOR PREPARING TRANSGENIC PIGS RESISTING PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME

摘要

A method for preparing transgenic pigs resisting porcine reproductive and respiratory syndrome (PRRS) is provided. The method comprises: preparing transgenic cells containing DNAs encoding shRNAs which target ORF1b, ORF5, ORF6 or ORF7 of porcine reproductive and respiratory syndrome virus (PRRSV); using the transgenic cells and isolated oocytes as donor cells and recipient cells respectively, obtaining cloned embryos by nuclear transplantation surgery; grafting the cloned embryos into uteri of domestic animals to initiate pregnancy by non-surgery method, and obtaining transgenic pigs. The transgenic pigs obtained by the method of the present invention have prominent capacity to resist PRRS.