Method and system for multiplex genetic analysis

摘要

A method for sequencing a plurality of target nucleic acid molecules using an apparatus, comprising:a sample holder configured to separate and confine a plurality of source points each comprising a single one of the target nucleic acid molecules, a fraction of a nucleic acid molecule, or a nucleic acid polymerizing enzyme molecule;a light source configured to direct excitation light toward the sample holder at an angle with respect to a normal of the sample holder, the excitation light illuminating the source points and causing the source points to fluoresce;at least one detector; andan optical assembly configured to collect fluorescent signals from illuminated source points to form images of the source points on the at least one detector.