Method of obtaining recombinant mammalian myeloma cell lines adapted to growth in serum- and protein-free media

摘要

The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval for each cell line. The invention also relates to mammal cell lines which grow in a stable manner in a medium free of serum and proteins over at least 40 generations. The invention further relates to clones which express anti-CD6 T1hT and EGFhR₃ antireceptor humanised antibodies and the anti-CD₃ T_3Q chimeric antibody as well as fragments of said antibodies.