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Probe for analyzing a polymorphism in the beta2AR gene, reagent, and obesity gene analysis method
Probe for analyzing a polymorphism in the beta2AR gene, reagent, and obesity gene analysis method
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机译:beta2AR基因多态性分析探针,试剂和肥胖症基因分析方法
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摘要
Primer sets for amplifying target regions containing sites to be detected in the obesity gene (the ß2AR gene, the ß3AR gene, and the UCP 1 gene) by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers composed of the base sequences of SEQ ID NO: 9 or SEQ ID NO: 109, SEQ ID NO: 25, and SEQ ID NO:43 as well as reverse primers composed of the base sequences of SEQ ID NO: 18, SEQ ID NO: 30, and SEQ ID NO: 63, respectively. The use of these primer sets makes it possible to specifically amplify a target region including a site where a polymorphism to be detected is generated in the ß2AR gene, the ß3AR gene, and the UCP1 gene, in the same reaction solution at the same time.
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机译:提供了通过基因扩增方法扩增在肥胖基因(ß2AR基因,ß3AR基因和UCP 1基因)中含有待检测部位的靶区域的引物组,其中该引物组可以特异性地扩增该区域。使用三对引物组,包括由SEQ ID NO:9或SEQ ID NO:109,SEQ ID NO:25和SEQ ID NO:43的碱基序列组成的正向引物以及由该碱基序列组成的反向引物分别为SEQ ID NO:18,SEQ ID NO:30和SEQ ID NO:63的序列。这些引物组的使用使得可以在同一反应溶液中同时扩增在ß2AR基因,ß3AR基因和UCP1基因中产生待检测多态性的位点的靶区域。
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