Probe for detecting mutation in exon 12 of NPM1 gene and use thereof

摘要

A probe for detecting a mutation(s) in exon 12 of the NPM1 gene, comprising at least one fluorescently labeled oligonucleotide selected from the group consisting of P5 and P5' below:(P5) an oligonucleotide comprising a nucleotide sequence complementary to a nucleotide sequence of 12 to 50 consecutive nucleotides containing nucleotides 145 to 156 in SEQ ID NO:2 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 145 is a cytosine labeled with a fluorescent dye; and(P5') an oligonucleotide comprising a nucleotide sequence complementary to a nucleotide sequence of 12 to 50 consecutive nucleotides containing nucleotides 145 to 156 in SEQ ID NO:2 or a sequence which hybridizes with the nucleotide sequence in SEQ ID NO:2 under stringent conditions, wherein the nucleotide corresponding to the nucleotide at position 145 is a cytosine labeled with a fluorescent dye.