GENETIC METHOD FOR DISTINGUISHING COLOUR VARIANTS IN STICHOPUS JAPONICUS SELENKA AND AN ALLELE-SPECIFIC PRIMER USED FOR THE SAME

摘要

PURPOSE: A genetic method for distinguishing colour variant in Stichopus japonicus Selenka and to improve accuracy of distinguishing.;CONSTITUTION: A genetic method for distinguishing colour variant Stichopus japonicus Selenka using allele-specific PCR comprises: a step of isolating genome DNA of HSP70 from variants in Stichopus japonicus Selenka; a step of adding a primer set of sequence numbers 2 and 3 and an allele-specific primer of sequence number 4 to the isolated DNA for performing PCR; and a step of determining the species. The primer of sequence number 4 is used for allele-specific PCR amplification to distinguish the color of Stichopus japonicus Selenka.;COPYRIGHT KIPO 2012