PURPOSE: A method for environmental health risk assessment by evaluating ecotoxicological reaction is provided to lower misdiganosis. CONSTITUTION: An assessment method is performed using: two or more among survival rate, growth rate, adult emergence, sex ratio and emergence period in Chironomus; two or more among survival rate, growth rate, regeneration rate, sex ratio and brooding period in Branchinella; and two or more among survival rate, growth rate, sex ratio and brooding rate in Tigriopus. Chironomus is Chironomus plumosus or Chironomus riparius. Branchinella is Branchinella kugenumaensis. Tigriopus is Tigriopus japonicus s.
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