MAGNETIC NANOPARTICLES SURFACE-MODIFIED WITH PHOSPHOCHOLINE, PREPARING METHOD OF THE SAME, AND SEPARATING METHOD OF PROTEIN USING THE SAME

摘要

PURPOSE: A magnetic nanoparticles which are surface modified with phosphocholine, a formation method thereof, and a protein isolation method using thereof are provided to simplify isolating process in order to easily isolate using magnetic nano particles which is surface modified with the choline phosphate and a magnet. CONSTITUTION: A magnetic nanoparticles which are surface modified with phosphocholine comprises magnetic nano particles and materials having choline phosphate group. The choline phosphate is combined with magnetic nano particle surface and is exposed. A formation method of material having choline phosphate comprises a step of radical polymerizing monomers having choline phosphate groups which is represented by the following structural formula at surfaces of the magnetic nano particles. The structural formula 1 is Choline phosphate -X-R. A isolation method of C-reactive protein comprises next steps: adding magnetic nanoparticles which are surface modified with choline phosphate to sample solution containing the C-reactive protein; forming a complex of the C-reactive proteins and magnetic nano particles; isolating the composite from the sample solution by using the magnet; and isolating the C-reactive protein from the isolated composite by using a dissociating agent.