Enzymatic production method for chitooligosaccharide and chitosan oligosaccharide using nano-particulated chitin and chitosan

摘要

PURPOSE: a kind of to prepare chitooligosaccharide- or chitosan oligosaccharide is provided to increase reaction speed and production low cost and prepare chitooligosaccharide- or chitosan oligosaccharide. ;CONSTITUTION: a kind of method that enzymetic prepares chitooligosaccharide- or chitosan oligosaccharide includes: to be put into the chitin of drying steps or chitosan is youth's head mill and to have crushed in size be 100-300 mesh with chitin or chitosan; Step is put into pulverizer and is crushed to ACM sizes 5,000-10,000; It introduces dry or wet ball mill with pulverized material is placed in and has crushed size 50-20,000nm. This method is executed, including uses fungi aspergillus strain, Muco kinds, Penicillium species, remote rattan and fabulous chitinase, chitosan enzyme, chitin or chitosan nano particle. ;The 2013 of copyright KIPO submissions;[Reference numerals] (AA) chitooligosaccharide- production yield; (BB) reaction time; (CC) control group; (DD) nanoparticle