N-terminal fragment of yellow fluorescent protein-fusion yeast library for in vivo analysis of protein-protein interaction

摘要

PURPOSE: A yeast strain library system for analyzing protein-protein interaction in yeast cell at genome-wide level is provided to quickly analyze the interaction of whole protein of yeast. CONSTITUTION: A gene recombinant Saccharomyces cerevisiae strain library contains yellow fluorescence protein N-terminal fragment gene which is operably linked to C-terminal on a genome DNA of Saccharomyces cerevisiae. A recombinant vector for substituting epitope tag has a sequence of sequence number 1 or 2.