High yield production methods for cross-linking bond bridged dimer and multimer by increasing the bond bridge formation utilizing the complex of multiple monomers and tandem-repeat chain of monomer specific binding group

摘要

The present invention body adhesive in the adhesive specificity in the monomer repeat - a plurality monomer complex-repeat chain created by the adhesive agent repeated chain and various monomers sikieo produce a repeated chain, and bonding the plurality of monomers in a repeating chain connection by having made, facilitating the formation of crosslinking between the monomers within the complex, as a large number of monomers for the production method of the oligomer attached to the cross-coupled, ; More specifically, the present invention sikieo bonding a large number of protein monomers in the production of adhesive protein repeat chain recombinant protein adhesive protein having an bond specificity to protein monomers are repeatedly connected, and the repeating chain protein to repeat chain and various monomers repeating chain made by - by making a monomer complex number created, facilitating the formation of crosslinking between the monomers within the complex, as a large number of monomers for the production method of the oligomer attached to the cross-coupled, ; More specifically, the present invention sikieo bonding a large number of protein monomers in the production of adhesive protein repeat chain recombinant protein adhesive protein having an bond specificity to protein monomers are repeatedly connected, and the repeating chain protein to repeat chain and various monomers repeating chain made by - by making a monomer complex number created, facilitating the formation of a disulfide bond between the cross-linking monomer in the composite, as a large number of monomers for the production method of the oligomer attached to the disulfide bond cross-linked, ; More specifically, the domain Ⅲ (Fab bonding domain) of protein G (protein G), Streptococcus (Streptococcal) is repeated - with an adhesive frame (binding matrix) recombinant repeated chain protein which is connected, the domain Ⅲ repeated chain protein sikieo to bond the plurality of antibody protein monomers creating a repeated chain with multiple antibody monomer complex, by facilitating the formation of disulfide cross-linking between the antibody monomers in the complex, a large number of monomers dimers and large amounts associated with disulfide bond cross-linked It relates to a method for producing a sieve. ; The method of the present invention can be effectively used for mass production of disulfide bond cross-linked dimers by yield improvement of up to 200 fold compared to conventional refolding method of the disulfide bond cross-linked dimer compared to refolding (refolding) methods known in the existing . ; Monomers, dimers, multimers, repeating chain, recombinant proteins, repeating chain-monomer complex number, crosslinked, crosslinking, crosslinking disulfide bonds.