Use of Near-Infrared Reflectance Spectroscopy for Estimating Caffeine and Individual Catechines Contents in Green Tea Leaves

摘要

The present invention relates to an individual content of simultaneous measurement of green tea catechins and caffeine, and more particularly measuring a near-infrared absorption spectra associated with the individual tea catechins and caffeine green tea component is irradiated with near-infrared light to green tea powder sample in any amount; Step that separates the green tea catechins, caffeine and green tea associated discrete near-infrared absorption spectra in the spectral content measurement for spectral calibration and validation of the individual tea catechins, caffeine and green tea; Step in developing a green tea caffeine and green tea catechins each individual calibrations calibration measurements are separated by the content of caffeine and green tea to get the raw spectra of the individual tea catechins and their derivatives related to near-infrared absorption spectrum by performing a statistical analysis and mathematical treatment; And the green tea caffeine content measurement and correction of the calibration equation for compensation spectrum of individual tea catechins and cross-validation, and the green tea caffeine content and spectrum measurements for validation of individual tea catechins to verify the suitability of the proven best calibrations relates to a method comprising the steps of selecting a calibration equation. According to the present invention, caffeine and green tea can be measured accurately in a short time at the same time the content of the individual green tea catechins, which can be particularly expected to be a more effective means to standardize the quality of the tea plant breeding programs with the Green Tea. ;