Method for Differentially Detecting a Multimeric Form from a Monomeric Form of Multimer-Forming Polypeptides Through Three-Dimensional Interactions

摘要

The present invention is a multi-bots from raw sample-capture on the surface of the present invention relates to methods and kits for them to discern detect multi meohyeong from the monomer type for forming the polypeptide, and more particularly (a) a solid phase of the carrier (solid phase carrier) as preparing a capturing antibody complex, said capture antibody is the multi-Merced-antibody three-dimensionally by combining a carrier and binding to one particular epitope of forming polypeptide; (B) a step of preparing a detection antibody, if an epitope the detection antibody which recognizes the detection antibody bound to the epitope that the position that causes the steric hindrance (steric hindrance) by the capture antibody binds to an epitope of the capture antibody and features; (C) the carrier in the raw sample - reacting a capture-antibody complex and the detection antibody at the same time; And (d) a carrier-capture antibody-directed to a method for detecting a fractional multi meohyeong from the monomer type for forming polypeptides and kits for this multi-meohyeong multi-Merced comprising the step of detecting the detection antibody complex. ;