TEST SYSTEM FOR DETERMINING circulating immune complexes

摘要

FIELD: medicine.;SUBSTANCE: for the purpose of detecting a level of circulating immune complexes (CICs), a precipitate of human blood serum in polyethylene glycol is prepared, centrifuged and dissolved. Total CIC level is determined either by optical density at wave length 280 nm and 260 nm, or by Lowry method; a complement-binding CICs are detected. The CIC precipitate is prepared with using a buffer containing 10% PEG 3350 in the ratio 1:1; the serum is incubated for 10 min at room temperature. The complement-unbinding CICs are detected as a difference between total CIC level and the complement-binding CIC. The group of inventions also concerns a test system for detecting the CIC level in human blood serum. The test system contains a CIC precipitation buffer representing 10% PEG in 0.01 M Tris-HCl-buffer, pH 7.4, as well as a dissolution buffer for the CIC precipitate representing 0.01 M Tris-HCl-buffer, pH 7.4, containing 0.15 M NaCl and a calibration solution containing human serum albumin in the concentration of 6 g/l in 0.01 M Tris-HCl-buffer, pH 7.4, containing 0.15 M NaCl.;EFFECT: inventions enable higher accuracy of blood serum CIC evaluation, provided in-depth study in immune, autoimmune and oncological diseases, and controlled effectiveness of the therapy.;2 cl, 3 tbl