METHOD FOR STANDARTISATION OF CONTROL SEED STRAIN PASTEURELLA MULTOCIDA

摘要

FIELD: medicine.;SUBSTANCE: invention concerns a method for standartisation of control seed strain of chicken cholera agents (Pasteurella multocida). The presented method involves cloning of the S- or M-form Pasteurella strain, gradual passaging by intramuscular, and then intranasal introduction in a susceptible organism of weight about 350 g; in 3 hours following after the death of every infected body, the microorganism is respectively isolated into physiological saline, and sealed by into 0.5-1.0 ml Pasteur pipettes with the Pasteurella strain isolated by the intramuscular passage being used over a period of 15-20 days as a by-product for the intranasal passage, while the Pasteurella strain isolated by the intranasal passage is used 2-3 days following the isolation procedure during the next 3 days as a reduction for producing a 10-hour (9 h at 37 and 1 h at 20°C) broth culture of encapsulated Pasteurellas.;EFFECT: invention enables producing the first-generation culture of encapsulated Pasteurellas recovered from the body with a specific microbial cell titre with these cells to be used for producing biopreparations.;15 dwg, 4 ex