METHOD FOR NONCOVALENT IMMOBILISATION OF LIPOPOLYSACCHARIDE Shigella flexneri 2a ON SOLID HYDROPHOBIC CARRIER

摘要

FIELD: chemistry.;SUBSTANCE: method involves passing a solution of lipopolysaccharide Shigella flexneri 2a in a 0.05 M triethylammonium acetate buffer at pH 4.7, which contains 10% n-propanol, through a column with a hydrophobic carrier which is Octyl Sepharose CL-4B, and then washing the column with the same buffer solution.;EFFECT: method can be used to produce carriers which enable extraction and purification of specific antibodies to lipopolysaccharides using affinity chromatography.;1 ex