MUS MUSCULUS CULTIVATED HYBRID CELL STRAIN 11D6 PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO LIPOPOLYSACCHARIDE FRANCISELLA TULARENSIS

摘要

FIELD: medicine.;SUBSTANCE: hybridoma strain is produced by immunization of BALB/c mice. The mice are immunised by common technique by double subcutaneous introduction of LPS Francisella tularensis 15/10. On the third day following the last booster injection, splenocytes of the immune mice are hybridised (1×10⁸ cells) and mice myeloma cells RZ-X63 Ag/8-653 (1×10⁷ cells). Polyethylene glycol (Sigma, the USA) is used as a fusion agent. The hybridisation is followed by hybridoma selection, screening, cloning and cryopreservation. The hybridoma 11D6 under the invention produces monoclonal antibodies (MCA) to LPS F. tularensis with titre making in a culture fluid 1:1000, in immunoascitic fluid 1:100000.;EFFECT: hybridoma produced MCAs are high specific to LPS Francisella tularensis and applicable for engineering test systems for rabbit disease agent detection.;7 dwg, 6 tbl, 7 ex