METHOD FOR PREPARING ERYTHROCYTE SUSPENSION FOR MEASURING ERYTHROCYTE MEMBRANE DEFORMABILITY

摘要

FIELD: medicine.;SUBSTANCE: invention describes a method for preparing an erythrocyte suspension for measuring erythrocyte membrane deformability involving venous blood sampling, erythrocyte washing and resuspension, differing by the fact that 3-fold erythrocyte washing is performed; each washing cycle is performed in a refrigerate centrifuge at 2700 rpm for 8 minutes at temperature 36.6°C in the low ionic strength solution Liss of pH 7.4 with a second erythrocyte washing cycle comprising the low ionic strength solution added with trypsin in the concentration of 2 mg/ml; and trypsin is removed during a third erythrocyte washing cycle. Each erythrocyte washing cycle is followed by removing a supernatant and adding the low ionic strength solution 5.0 ml in each erythrocyte suspension 1.0 ml, and the third erythrocyte washing cycle is followed by resuspending the washed erythrocytes 0.1 ml in the low ionic strength solution 1.9 ml.;EFFECT: higher effectiveness of the method for preparing the erythrocyte suspension with minimally changed membranes for measuring deformability of the latter.;1 ex