Detection of living, a circulating or disseminated cells or cell components in the blood or bone marrow after filtration of blood

摘要

A method for the detection of circulating cells in a sample of a body fluid, comprising the following steps: a) filtration of the sample of body fluid through a porous membrane b) transfer of the membrane with the filter residue is located on it, the cells in a cell culture vessel, wherein a surface of the cell culture vessel is coated with a first antibody, which is directed against a first cell-specific marker is c) incubating the diaphragm in the cell culture vessel, with a cell culture medium for a predetermined period of time, wherein of the cells may be present is emitted from the first antibody cell-specific marker is to be bound; d) removing of the membrane with the cells to be located thereon as a filter residue from the cell culture vessel; e) detecting the bound cell-specific marker on the surface coated with the first antibody.