Solubilizing and clarifying water-based cooling lubricants, comprises adding clarification reagent, carrying out immediate clarification without heating emulsion and carrying out measurement in clear, transparent liquid with optical method

摘要

Solubilizing and clarifying the water-based cooling lubricants, which are present as an emulsion for quantitatively determining the content of microorganisms, and their metabolic activity, comprises: adding a clarification reagent comprising organic and/or inorganic salts together with non-ionic surfactants in the defined concentration and a composition as a formulation of the cooling lubricant; carrying out immediate clarification at room temperature without heating the emulsion; and carrying out a measurement in a clear, transparent liquid with optical- or physical methods. Solubilizing and clarifying the water-based cooling lubricants, which are present as an emulsion for quantitatively determining the content of microorganisms, and their metabolic activity in the area of interest in real time, comprises: adding a clarification reagent comprising organic and/or inorganic salts together with non-ionic surfactants in the defined concentration and a composition as a formulation of the cooling lubricant; carrying out immediate clarification at room temperature without heating the emulsion; and carrying out a measurement in a clear, transparent liquid with optical- or physical methods, including flow cytometer in real time.