Two-dimensional liquid chromatography methods, systems and software programs

摘要

A method of providing two-dimensional liquid chromatography (in a system 200 comprising a first liquid chromatograph 210 coupled with a second liquid chromatograph 220) comprises controlling (via controller 290) an injection event of injecting an output of the first liquid chromatograph 210 into the second liquid chromatograph 220 in relation to a state (600, 610, 620, 630) of the second liquid chromatograph 220. The state of the second liquid chromatograph may represent a specific mechanical configuration of or within the second liquid chromatograph (e.g. a direction of movement or a position of a piston, or a cycle wherein the primary piston (420, 510) does not supply fluid to the secondary piston (435, 530). This may allow an increase in the reproducibility of the second dimension separation, as provided by the second liquid chromatograph, by relating the injection to the state of the second liquid chromatograph. The second liquid chromatograph may comprise reciprocating pumps 265 and 267.