New microbe, inurinaze, inulin saponifier, production manner of inulo-oligosaccharide and production manner of inurinaze

摘要

P To provide a means for easily producing an inulo-oligosaccharide and especially inulo-tetraose by using inulin as a raw material. PSOLUTION: The Microbacterium sp. YK-01 (FERM P-21593) strain and the inulinase originated from the Microbacterium sp. YK-01 (FERM P-21593) strain are provided. The inulinase has the following physicochemical properties: (1) forming a linear inulo-oligosaccharide by acting ON the inulin and wherein the amounts of the products are in order of inulo-tetraose inulo-triose inulo-biose and fructose; (2) having the optimum pH of 6-8.5; (3) having the optimum temperature of 45-55° C; (4) stable at ≤ 50° C; and (5) having about 128,000 molecular weight measured by SDS-polyacrylamide gel electrophoresis. PCOPYRIGHT: (C)2010 and JPO& INPIT