Fluorescent detection device, sample electrolysis cell and fluorescent method of detection for fluorescent detection

摘要

To enable highly sensitive fluorescence detection at low cost by using a simple optical system in a fluorescence detecting device. [Constitution] Excitation light (L 0) from a light source (32) is caused to enter a finely apertured thin metal film (12), having fine apertures (12a) with diameters less than or equal to the wavelength of the excitation light (L 0), formed ON a surface of a substrate (11) different from a light incident surface thereof and through the substrate (11), to cause near field light to be generated at the fine apertures (12a). Fluorescent labels included in a sample liquid S supplied to contact the thin metal film (12) are caused to emit light by the near field light and by surface plasmon which are induced in the finely apertured thin metal film (12) by the near field light. Fluorescence (L f) emitted by the fluorescent labels is detected with a detector (30). Fluorescent particles (F) having a plurality of fluorescent pigments (15) enveloped in a transmitting material (16) that transmits both the excitation light (Lo) and the fluorescence (L f) are employed as the fluorescent labels.