PLASMID-TYPE VECTOR, METHOD FOR DETECTING GENE PROMOTER ACTIVITY AND ASSAY KIT

摘要

PROBLEM TO BE SOLVED: To detect activity of a gene promoter in high sensitivity.SOLUTION: The plasmid-type vector 1 has a gene promoter 10. A reporter gene 21, an internal ribosome entry site (IRES) 23, a replication initiation protein gene 25, and a transcription termination signal sequence 27 are arranged at the downstream of the gene promoter 10. The reporter gene 21 encodes a reporter protein for visualizing activity of the gene promoter 10. The replication initiation protein gene 25 encodes the replication initiation protein. The IRES 23 is arranged between the reporter gene 21 and the replication initiation protein gene 25. The transcription termination signal sequence 27 encodes a signal for terminating transcription of the reporter gene 21 and the replication initiation protein gene 25. The plasmid-type vector 1 has a replication initiation sequence 30. The replication initiation sequence 30 is recognized by the replication initiation protein.