The silica nano grain which is obtained by the production manner and aforementioned production manner of the silica nano grain which contains the organicity pigment numerator possessing the carboxyl group, the indicator reagent null following

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a silica nanoparticle, in which the efficiency is improved when a functional organic molecule having a carboxy group, such as a fluorescent pigment molecule and a light absorbing pigment molecule, is fetched in the silica nanoparticle, and to provide the silica nanoparticle to be produced by the producing method, a method for fetching the functional organic molecule and a labeling reagent which is excellent in reproducibility of measured results and allows an ultramicro-target substance to be analyzed quantitatively with high sensitivity.;SOLUTION: The method for producing the silica nanoparticle containing the functional organic molecule having at least one carboxy group includes: a step (a) of amide-bonding the functional organic molecule having at least one carboxy group and at least one active ester group to at least one amino group of organoalkoxysilane; and a step (b) of hydrolyzing both of an amide-bonded compound obtained at the step (a) and tetraalkoxysilane and subjecting the obtained organosilanol and silanol to a polycondensation reaction to prepare the silica nanoparticle containing the functional organic molecule.;COPYRIGHT: (C)2010,JPO&INPIT