cells, and method for producing optically active amino acid or optically active N- carbamyl amino acids 5-substituted hydantoin racemase, DNA encoding the same, recombinant DNA, transformed

摘要

Hydantoin racemase novel, and the invention provides a process for producing an optically active amino acid or optically active N- carbamylamino acid using the same. As a feature of the present invention, a novel hydantoin racemase that has been isolated and purified Bacillus species from (Bacillus sp.) KNK519HR strain, the gene encoding the hydantoin racemase, a recombinant plasmid containing the gene, and is introduced to the hydantoin racemase gene Transformants were the like. In another aspect of the invention, by allowing the presence of the hydantoin racemase simultaneously with N--carbamyl amino acid amide hydrolase and hydantoinase the 5-substituted hydantoin compound, the process for producing an optically active amino acid or optically active N--carbamyl amino acid and the like.