Topic The presence of the signal as an index can detect the target unique ligand simply and accurately the probe of the single molecular type which is offered.Solutions It is the monad type bioluminescence visualization probe which detects the target unique ligand in the raw cell, when on the both ends of the ligand connection molecule which changes the three-dimensional structure when the ligand is connected, the N terminal side polypeptide (N-LE) and C terminal side polypeptide (C-LE) which divide the luminous enzyme (LE) respectively are connected are approached, the ligand connects to the ligand connection molecule only, self compliment doing N-LE and C-LE reversibly, the luminous signal is given out. As for the ligand connection molecule, for example, LBD it is the fusion molecule which connects with domain (LBD interaction domain) or the peptide which operates mutually with ligand connection domain (LBD) and the connection of the ligand. Selective figure Figure 1
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