Proteins with diaphorase activity

摘要

The object of the present invention is that even when it is subjected to a heat treatment given to provide a mutant diaphorase diaphorase activity residual rate was improved relative to the wild type. The present invention, facilities 80 ℃ and a protein having diaphorase activity comprising an amino acid sequence that introduce amino acid substitutions in specific amino acid sequence of wild-type diaphorase represented by SEQ ID NO: 1 in the Sequence Listing, a heat treatment of 10 minutes relates to a protein diaphorase activity residual ratio in the case of the have improved as compared to a protein consisting of the amino acid sequence shown in SEQ ID NO: 1.