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METHOD FOR CONSTRUCTING LIBRARIES OF NON-STANDARD PEPTIDE COMPOUNDS COMPRISING N-METHYL AMINO ACIDS AND OTHER SPECIAL (NON-STANDARD) AMINO ACIDS AND METHOD FOR SEARCHING AND IDENTIFYING ACTIVE SPECIES
METHOD FOR CONSTRUCTING LIBRARIES OF NON-STANDARD PEPTIDE COMPOUNDS COMPRISING N-METHYL AMINO ACIDS AND OTHER SPECIAL (NON-STANDARD) AMINO ACIDS AND METHOD FOR SEARCHING AND IDENTIFYING ACTIVE SPECIES
A method for screening a non-standard peptide compound in the peptide library that binds to the target substance, comprising the steps: (i) preparing a non-standard peptide library wherein a special (non-standard) amino acid is randomly incorporated into the peptide sequence by a cell-free (in vitro) translation system comprising a tRNA acylated by a special (non-standard) amino acid; (ii) bringing the obtained peptide library in contact with a target substance; and (iii) selecting a non-standard peptide that binds to the target substance as an active peptide.
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