Mobility Controlled Single Macromolecule in Nanofluidic System and its Application as Macromolecule Sequencer

摘要

In one embodiment, the present application discloses a microfluidic device comprising at least one nanochannel, in part, configured to receive a macromolecule, at least one external electrodes configured with the nanochannel, that is further configured to provide contact of the macromolecule with an enzyme to cleave the macromolecule to form a fragment of the macromolecule. Also disclosed are methods of using the microfluidic device for detecting and identifying a property of the fragment of the macromolecule.