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PROBE AND PRIMER FOR TUBERCLE BACILLUS DETECTION, AND METHOD OF DETECTING HUMAN TUBERCLE BACILLUS THEREWITH
PROBE AND PRIMER FOR TUBERCLE BACILLUS DETECTION, AND METHOD OF DETECTING HUMAN TUBERCLE BACILLUS THEREWITH
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机译:用于检测结核杆菌的探针和底漆,以及由此检测人结核杆菌的方法
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摘要
An object of the present invention is to provide a novel primer and prove for detecting tubercle bacillus capable of avoiding false positive, and an easy-to-use, rapid and high-sensitivity method for detecting human tubercle bacillus (Mycobacterium tuberculosis) using the same. The present invention relates to an oligonucleotide comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis, a primer and a probe containing the oligonucleotide for detecting Mycobacterium tuberculosis, and a method for detecting Mycobacterium tuberculosis using the primer and the probe.
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机译:本发明的目的是提供一种新颖的引物和用于检测结核杆菌的证明,其能够避免假阳性,以及一种易于使用,快速且高灵敏度的检测人结核杆菌的方法(结核分枝杆菌< / I>)。本发明涉及一种寡核苷酸,其包含SEQ ID NO:1,SEQ ID NO:2,SEQ ID NO:3,SEQ ID NO:4,SEQ ID NO:1中描述的核酸序列的一部分或全部序列。 5,SEQ ID NO:6,SEQ ID NO:7或SEQ ID NO:8或其互补序列的一部分或全部序列,其中寡核苷酸具有与IS6110基因的核酸序列杂交的特性在结核分枝杆菌中 I>引物和包含用于检测结核分枝杆菌 I>的寡核苷酸的探针,以及使用引物和探测。
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