Fully human antibody to human TNFα, molecular evolution and use thereof

摘要

The present invention relates to the discovery and improvement of proteins that can specifically bind with human TNFa (Tumor Necrosis Factor alpha), the proteins are full human anti human TNFa monoclonal antibodies derived from human B cells. This invention disclosed the anti human TNFa monoclonal antibodies, their amino acid sequence of light chain comprising SEQ ID NO:8, and that of heavy chain comprising SEQ ID NO:16 or SEQ ID NO:18. This invention disclosed the coding genes of these antibodies, their Fab molecules, and their potential application to treat clinically the diseases related to TNFa, such as inflammation diseases.