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System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
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机译:对量子点光学编码技术产生的多路复用荧光光谱信号进行解卷积的系统和方法
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摘要
A method deconvolves a multiplexed fluorescence spectral signal into its component single-color fluorescence spectra emitted by contributing fluorophore types. A spectral database contains a parameter set for each of the component spectra. Optical codes are received as the multiplexed signals. A sliding window technique estimates a number and location of local peaks for the component spectra. Allowable variation ranges are assigned for the corresponding parameter sets. Each multiplexed signal is deconvolved into a sum of parametric values to generate a list of its component spectra. Encoded and received optical codes, in the form of multiplexed signals, are decoded and identified by their component spectra and contributing fluorophore types. A system for deconvolving the multiplexed signals includes a spectral database containing the parameter sets, a detection element receiving the optical codes, and a signal processor encoded to analyze and deconvolve the multiplexed signals.
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