Gene cassette of anchorage of the mer - R, tape expressu00e7o - anchor of mer - R, plasmideo recombinant bacterial lineage transgu00canica, use of said gene, use of this lineage in processes of environmental biorremediau00c7u00e7o

摘要

Invention patent "gene cassette of anchorage of the mer - R, tape expressu00e7o - anchor of mer - R, plasmideo recombinant bacterial lineage transgu00canica, use of said gene, use of this lineage in processes of environmental biorremediau00c7u00e7o".The present invention relates to the construction and insertion of a vector of broad spectrum for gram negative bacteria, containing a gene sequence that can be expressed, allows anchoring of a protein chelating ions of mercury on the cell surface of gram negative bacteria.For this, the regulatory gene was isolated from plasmid pmol30 Merr of cupriavidus metallidurans, line ch34 (SEQ. ID No. 1) and inserted into cloning vector pgemt, resulting in the plasmid pgemt Hg (SEQ. ID No. 2).The expression vector containing the sequence corresponding to the tape to the expression and anchoring of heterologous proteins in gram negative bacteria under the control of the Da pan (SEQ. ID No. 3), derived from the plasmid pcm2, was obtained by polymerase chain reaction (PCR). The Merr gene was fused expression vector, amplified, resulting in the construction of the plasmid pcmhg (SEQ.ID No. 4). In addition, the present application provides mutant strains of gram negative bacteria containing said recombinant plasmid, method of production, beyond reporting the possible use of transgenic strains for the absorption of ions of mercury in environmental bioremediation processes.