Stereoselective process for the preparation of (1S, 2S)-1-halo-2-hydroxy-3 (protected) amino-4-substituted butanes

摘要

The present invention relates to a stereoselective process for the preparation of (1 S, 2S)-1-halo-2-hydroxy-3 (protected) amino-4-substituted butanes represented by the general formula I wherein Hal represents halogen, R is selected from the group consisting of alkyl, substituted alkyl, aryl and substituted aryl and Ri1 represents a protecting group for the amino function comprising contacting a (3S)-l-halo-2-oxo-3- (protected) amino-4-substituted butane represented by the general formula II wherein Hal, R and Ri1 are as defined above with a microorganism capable of catalyzing the stereoselective reduction of the compound represented by the general formula II wherein said microorganism is selected from the group consisting of Rhodococcus erythropolis ATCC 4277, Rhodococcus erythropolis DSM 6971 and Rhodococcus sp. ATCC 21227, Rhodococcus erythropolis ATCC 27854 and Brevibacterium sp. ATCC 19653 under conditions such that said reduction is effected, and recovering said compound represented by the general formula I.