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Isolated disc stem cells and disc progenitor cells obtained from the nucleus pulposus, methods for producing, methods of using them and compositions comprising same
Isolated disc stem cells and disc progenitor cells obtained from the nucleus pulposus, methods for producing, methods of using them and compositions comprising same
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机译:从髓核获得的分离的椎间盘干细胞和椎间盘祖细胞,生产方法,使用它们的方法以及包含它们的组合物
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摘要
Provided is an ex-vivo method of producing an enriched cell population comprising the steps of plating a heterogeneous population of nucleus pulposus cells in a media at low cell density; growing the cells under conditions that inhibit cell attachment; selecting free-floating in vitro spherical structures comprising disc stem cells, disc progenitor cells, or a mixture thereof; thereby producing an enriched cell population. An ex-vivo method of expanding a population of disc stem cells, disc progenitor cells, or a mixture thereof is also provided, as is an ex-vivo method of producing an artificial disc and methods of treating a subject having a damaged intervertebral disc.
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