PREPARATION AND CHARACTERIZATION OF THE RECOMBINANT MEMBRANE PROTEIN (MCA2) IN THE SF9 INSECT CELL LINE.

摘要

The present invention provides preparation and characterization of the recombinant membrane protein (MCA2) in the Sf9 insect cell line. The method provides the preparation of recombinant MCA2 in eukaryotic system. The eukaryotic expression enhances the level of post-translational modifications as well as the degree of multimerisation. Hence, the structure-function and oligomeric structure of recombinant MCA2 produced in the eukaryotic expression host resembles the WT protein. Purification of the recombinant protein is optimized using immobilized metal ion affinity chromatography and the muitimeric state of the recombinant preparation is also disclosed.