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SOLUBLE CYTOPLASMIC EXPRESSION OF HETEROLOGOUS PROTEINS IN ESCHERICHIA COLI
SOLUBLE CYTOPLASMIC EXPRESSION OF HETEROLOGOUS PROTEINS IN ESCHERICHIA COLI
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机译:大肠埃希菌中可溶性脂蛋白的细胞质表达
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摘要
Soluble variants of recombinant proteins produced in a prokaryotic host cell, where the high expression levels often cause the original proteins to aggregate into insoluble inclusion body aggregates. The variant polypeptides retain biological function while increasing protein solubility with comparable or higher recoverable levels of biologically active protein when expressed in a suitable expression host. Methods of identifying critical residues and substituting them are provided to produce the variants.
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