A NEW FLUORESCENT BEACON PROBE DIAGNOSTIC TOOL/KIT FOR MILECULAR DETECTION OF TRANSLOCATION T(9;22)/BCR-ABL1 IN LEUKEMIC PATIENTS AND METHOD OF WORKING FOR THE SAME

摘要

The present invention provides method for detecting chromosomal abnormalities in leukemic patients and particularly uses nucleic acid hybridization technique for detection of BCR-ABLl translocation/ t(9;22)(q34;qll) or the so called Philadelphia chromosome which is found in 95% of Chronic Myeloid Leukemia ,15-20% acute lymphoid leukemia and 5% of Acute Myeloid Leukemia patients . The invention focuses on an easy, inexpensive/pocket friendly, rapid and efficient detection of commonly found b2a2 transcript (RNA) of BCR-ABLl from peripheral venous blood of leukemia patients by using beacon based oligonucleotide fluorescent probe which detects the transcribed regions on both sides of the junction formed in the fusion gene upon translocation.