Methods for assaying and isolating cell lines which stably expressed a sweet/bitter/umami taste receptor

摘要

Disclosed is a method for isolating a cell; wherein said cell comprises an endogenous nucleic acid that expresses a sweet taste receptor T1R2 subunit and/or an endogenous nucleic acid that expresses a sweet taste receptor T1R3 subunit, wherein the method comprises the steps of: a. providing a population of cells; b. introducing into the population of cells a molecular beacon that detects expression of T1R2 and/or introducing into the cells a molecular beacon that detects expression of T1R3; and c. isolating cells that express a sweet taste receptor T1R2 subunit and/or sweet taste receptor T1R3 subunit.