PROCESS FOR THE PURIFICATION OF RECOMBINANT ALPHA 1-ANTITRYPSIN INVOLVING A STEP OF ANION EXCHANGE CHROMATOGRAPHY

摘要

The present invention relates to a method for obtaining highly purified recombinant alpha1-antitrypsin (rAAT) using anion exchange chromatography, wherein a composition comprising rAAT and at least one impurity originating from cultivation of cells used for generating of rAAT is loaded onto a column containing anion exchange material and the anion exchange chromatography is carried out with buffers containing phosphate ions and N-acetylcysteine.