DNA sequence, oligonucleotide primers, the DNA sequence with recognition sites for restriction enzymes NcoI and EcoRI, expression plasmid and a method for its obtaining, recombinant Escherichia coli strain TOP10F' and its method for obtaining and a method for producing thermostable SSB-like protein Nanoarchaeum Kin4-M equitans

摘要

the subject invention is estimated kodujacego gene sequence termostabilne ssb protein nanoarchaeum equitans kin4 - m.the subject of the invention is a dna sequence obtained by dna amplification using primers.containing the dna fragment kodujacy termostabilne protein ssb - similar nanoarchaeum equitans kin4 - m and expressive vector containing dna fragment wklonowane kodujacym protein ssb - similar nanoarchaeum equitans kin4 - m.the invention also includes the method for obtaining rekombinantowego strain of escherichia coli top10f the escherichia coli top10f? - pbadneqssb and manufacture termostabilnego protein ssb - comes closesome nanoarchaeum equitans kin4 - m.