A diagnostic test based on amino-modified nanogold coupled with the carboxy-modified nucleotides for identifying drug-resistant bacterial strains and the method for its preparation

摘要

the subject of the invention is a diagnostic test based on amino modified nanozlota sprzezonego with karboksy modified nukleotydami to identify lekoopornosci bacterial strains.which is made up of membrane nitrocelulozowej type lateral flow of natezeniu movement of analyte from 135 to 240 seconds / cm nasaczonej way ahead on points solution protein wiazacego biotyne in amounts of from 0.005 mg / mm2 to 0.1 mg / mm2 suspended in 10% aqueous solution of ethanol and reagent request detector 25. g amino modified nanoczastek au sprzeganych kowalencyjnie with probe modified hybrydyzacyjna lacznikiem karboksylowym in of 1 - 10 nm with 1 - 10 pmolami probe hybrydyzacyjnej of biotin.process for the production of a diagnostic test based on amino modified nanozlota sprzezonego with karboksy modified nukleotydami to identify lekoopornosci bacterial strains is,in the first stage membrane nitrocelulozowa type lateral flow of natezeniu movement of analyte from 135 to 240 seconds / cm nasacza's way ahead on points wiazacego biotyne in protein solution of 0.005 mg / mm2 to 0,1 mg / mm2 suspended in 10% aqueous solution of ethanol.in the second stage is inkubacje modified liaison aminowym nanoczastek gold of stezeniu 25? g / ml with the probe hybrydyzacyjna with free carboxyl group containing from 1 to 10 nm oligonukleotydu, after the sprzegania powstale nanoczastki au being concentration in process of spinning 14 400 x g for 30 minutes.the supernatant and the precipitate suspended rejects it at 100? l - 200? l deionised distilled water, then the solution is subjected to concentration again,the nanoczastek au suspended in 39 - 109. deionised distilled water and added to l 1? l solution biotynylowanej probe hybrydyzacyjnej zawierajacej 1 - 10 pmoli of the oligonucleotide.then in the third stage to 10 - 30? l the detector reagent adds to 10? l shaggy bacterial culture, mieszanine reactionary placed in bath water temperature in the 90 - 100u00b0c during 3 - 5 minthen leaves to ostygniecia to room temperature and 10 - 20? l mixture moving in with immobilizowanym reaction membrane bialkiem wiazacym biotyne.