METHOD TO DETERMINE INTENSITY OF LIPID PEROXIDATION OF BASIDIOMYCETES CULTURES

摘要

A method to determine the intensity of lipid peroxidation of basidiomycetes, which comprises obtaining a cultural filtrate, homogenizing the mycelium, adding to the mycelial homogenate and the cultural filtrate of solutions of trichloracetic acid and thiobarbituric acid and incubating the reaction mixture on a boiling water bath, cooling, centrifuging and spectrophotometric measurement of extinctions of test samples against control ones at a wavelength of =532 nm. Prior to the homogenization the mycelium is cooled, then the cooled mycelium is homogenized for three times at a temperature less than or equal to +5 °C, the 1time the mycelium, 2 after adding 2.0 ml of 22.5 % trichloracetic acid, 3 2.0 ml of 0.6 % solution of thiobarbituric acid, the mixture is incubated for 15 min, cooled up to +20 °C, centrifuged, extinction of test samples is measured against a control one (of 0.5 ml of distilled water) at wavelengths of 532 nm and 590 nm, the amount of the lipide peroxidation products reacting with thiobarbituric acid is calculated in nmol/g of absolutely dry mycelium biomass or per ml of the cultural filtrate.