bacterial consortium, bioelectroquímico device and a process for the simple and rapid estimate of the biological oxygen demand of the wastewater

摘要

A process for the rapid estimation of BOD wastewater beverage using a bioelectroquímico device in which the process comprises the following steps: a) providing a device comprising bioelectroquímico i) an immobilized microbial electrode; ii) a multimeter; and iii) a mobile workstation with a software installed configured to take the change in the current values ​​in real time and to manipulate the values ​​in the desired graphic displays; in which microbes form a bacteria consortium consisting of Aeromonas hydrophila (PTA-3751), Pseudomonas aeruginosa (PTA-3748), Yersinia enterocolitica (PTA-3752), Pseudomonas fluorescens (PTA-3749), Enterobacter cloacae (PT A- 10 3882); b) applying an external bias of -0.62 V to -0.67 V to the immobilized microbial bioelectroquímico electrode device; c) stabilize the electrode assembly in phosphate buffer 0.025 to 0.075 M at pH 6.4 to 7.2 for 30 to 45 minutes, followed by addition of glucose-glutamic acid (GGA) in phosphate buffer; d) observing the stability of the microbial membrane immobilized through a software that uses a set of stabilized device electrodes by measuring the change in oxygen concentration in terms of current covering a concentration range GGA used, ie 15 to 75 mg / l; e) calibrating the change in current for different concentrations GGA obtained from step (d) with conventional BOD values; f) replacing the solution GGA by fresh buffer phosphate and stabilize the assembly; g) adding the waste water drinks in phosphate buffer 0.025 to 0.075 M at pH 6.4 to 7.2 to maintain the percentage determined by the software; h) observing the change in the oxygen concentration in terms of current with the help of software; i) calculating the BOD values ​​using software with the help of a calibration curve by the change in current as shown in step (h)