Method for the detection and quantification of Mycobacterium avium subspecies for tuberculosis based on polymerase chain reaction in real time

摘要

Method for the detection and quantification of Mycobacterium avium subspecies mediantedos paratuberculosis chain reactions Polymerase competitive real-time, each with its own control internal deamplificación, characterized by the fact that detection and quantification deMycobacterium avium subspecies paratuberculosis is performed using two reaction systems chains polymerase independent real time, which detect two specific locus paraMycobacterium avium subspecies paratuberculosis: 900 and F57 respectively, using primers hydrolyzing ysondas having the following sequences.