Procedure for the determination of an in vitro dose of antibiotic by genetic regulatory mechanisms and corresponding diagnostic kit

摘要

said kit intended auna implementation kit for detecting and / or quantifying an antibiotic (C), belonging to the family of tetracyclines or lasvirginiamicinas present in a test sample, by a receiver (A), wherein in vitro, ie unexpressed in any biological or cellular system, characterized porqueconsiste the following reagents: - a receiver mode (a), a genetic repressor respectively tetracycline or virginiamycin, which present it property exhibiting at least a first site specific recognition for antibiotic (C) and unsegundo recognition site specific for a nucleotide sequence operator (B) corresponding alrepresor, the second site separate from the first site, and has the property that fixing nucleotide dichasecuencia (B) in the receiver (a) in said second specific recognition site is modulanegativamente by fixing aNTIBIOTIC or (C) in the receiver (A) in said first site reconocimientoespecífico, ie fixing said nucleotide sequence (B) occurs only in defijación absence of the antibiotic (C) in the receiver (A), - said sequence nucleotide (B), single or double stranded.