USE OF FUSION PROTEINS WHOSE N-TERMINAL PART IS A HIRUDIN DERIVATIVE FOR THE PRODUCTION OF RECOMBINANT PROTEINS VIA SECRETION BY YEASTS

摘要

The invention relates to an expression cassette of the form Px-Sx-Bn-(ZR)-Hir(AsmR)-protein (Y) - T, where Px is any promoter DNA sequence, selected insuch a way that optimal yields of the protein of interest become achievable;Sx is any DNA encoding a signal sequence or leader sequence which allowsoptimal yields; Bn is 1-15 amino acid codons or a chemical bond; Z is thecodon of a amino acid selected from the group comprising Lys and Arg; R is anArg codon or a chemical bond; Asm is a chemical bond or m amino aid codons,where m= 1-10; Hir is a DNA sequence coding for hirudin or a hirudinderivative which is at least 40 % homologous to natural hirudin; protein Y isa DNA sequence encoding any protein which can be produced in and secreted byyeast; T is an untraslated DNA sequence which is advantageous to theexpression.